LC/MS Fundamentals and Applications


Formats: Classroom, Live-on-the-Web, Self-Paced, and customized in-house
Length: Classroom - 3 days; Live-on-the-Web - Two 90-minute sessions per week for 6 weeks
Fee: $1,600 USD
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Fred Klink instructing the classLC/MS: Fundamentals and Applications is a comprehensive overview course that provides attendees with relevant, practical approaches that can be immediately applied in the lab. The theory of mass spectrometry and LC interfaces is presented to the extent necessary to understand operation of instruments but the real focus of this class is improving your ability to use LC/MS on a daily basis. This course is offered as a three-day classroom course or live-on-the-web over a six week period. You may also contract with us to present a private session of the course exclusively for your organization either in-person or on the web. In this case, some degree of customization of the course material is possible in order to address your specific needs and problems.

Who Should Attend

Researchers, practitioners, technicians, and others who are currently using LC, LC/MS, or plan to use LC/MS in the future, and those dealing with data produced by LC/MS. This course is designed to provide a practical overview. Chromatographers just embarking on the technique will gain insight on how to select the appropriate instrument for different applications, and those currently using LC/MS and its data will develop an appreciation for, and an understanding of, the complexities of the data generated.

How You'll Benefit from This Course:

  • Understand the operational fundamentals of liquid chromatography, mass spectrometry, and the commercially available LC/MS interfaces.
  • Receive an overview of LC/MS applications in pharmaceutical, chemical, and biotechnology industries plus information on forensic and toxicological applications
  • Learn how to modify existing HPLC methods to optimize results when moving to LC/MS.
  • Find out how to use LC/MS to obtain qualitative information about your samples; from simple molecular weight determinations to structural analysis through spectral interpretation.

Course Topics:

  • Introduction to LC/MS: overview and historical perspective
  • Review of atoms, isotopes, molecules and ions: how to think about these topics in order to better understand mass spectrometry
  • What is MS?: history, mass analyzer types, MS/MS and CAD
  • LC/MS Interfaces: historical interfaces, atmospheric pressure chemical ionization, electrospray and atmospheric pressure photoionization interfaces
  • Optimization of LC/MS methods: how mobile phase and matrix components can affect results; methods for determining and fixing optimization problems; how electrospray method development differs from APCI
  • Handling the data from LC/MS: both qualitative (spectral interpretation) and quantitative use of MS data is discussed
  • Applications review: drugs and metabolites, pharmaceuticals, environmental applications, natural products, protein/peptide analysis are presented
  • Bibliography of LC/MS; glossary of correct and incorrect terms

What attendees are saying...

"I was very pleased with the course. It fulfilled my goal of achieving a basic undderstanding LC/MS and GC/MS, which will be helpful in future method reviews and possible LC/MS operation. I also very much liked the comments for each slide. GREAT COURSE!" —Elizabeth Flynt, Chemist, U.S. EPA

" Perfect course, Thank you! It was exactly what I needed." —Ray Miller, Chemist, New Orleans, LA

Instructors: O. David Sparkman and Fred Klink