Learning Modules


Format: On-demand (through our UK partner, Separation Science)
Length: 45 minutes
Fee: $147 for 1 month unlimited access

Simple-to-use slide-by-slide video presentations with instructional voiceover, together with a comprehensive handout supporting the learning experience.

How Does Electrospray Work?     Register Today!

This 45 minute video lecture outlines the basic operational principles of an electrospray interface (ESI) as used for LC/MS analyses. While we often feel that “theory” is a waste of time and we ask instead for “practical” information, this course will satisfy both of those needs. Appreciating how this very complex interface works will help the analyst to better understand the various (and numerous!), settings that must be dealt with to optimize an LC/MS method. A basic familiarity with electrospray operating principles will also make it clear why certain HPLC mobile phase compositions and components are best for LC/MS.

How Will You Benefit from the Course?

  • Gain a more complete understanding of the operation of the electrospray interface
  • Have a “tool kit” for solving ESI problems
  • Be better able to discuss electrospray interfaces with vendors and service personnel
  • Have a foundation for learning detailed method development and optimization procedures

Developing Electrospray LC/MS Methods, Part 1      

Developing Electrospray LC/MS Methods, Part 2      

Perhaps more than any other analytical instrument, electrospray (ES) LC/MS method development requires the chemist to carefully proceed in a stepwise fashion and evaluate the results at each step. Each 45 minute video lecture in this two-part series will provide you with the basic information needed to begin successful method development for ES.

How You will Benefit from this Course

  • See the complete list of variables which effect ES LC/MS results
  • Understand the important concept of ion suppression, it’s causes and solutions
  • Learn about metal adduct formation and what to do about it
  • Gain insights into how the choice of mobile phase constituents for ES analyses must be made depending on the analyte compound and analytical goal of the method
  • Be better able to discuss electrospray results with colleagues, contract labs, and instrument service/support personnel